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目的:建立一种准确、快速、简便的检测去甲氯胺酮(NKET)的胶体金免疫层析检测技术。方法:采用柠檬酸三钠还原法制备一定粒径的胶体金溶液,并标记抗NKET单克隆抗体,将一定浓度的包被抗原(NKET-OVA)及羊抗鼠二抗IgG喷在硝酸纤维素膜上,制备胶体金免疫层析试纸条。结果:该试纸条检测阈值可达500 ng/ml,检测的准确性为97.2%,试纸条的稳定性和重复性良好。结论:采用该试纸条检测去甲氯胺酮特异性强,灵敏度高,而且简便快速,无需特殊仪器设备,在毒品滥用筛查工作中应用价值较大。
OBJECTIVE: To establish an accurate, rapid and simple colloidal gold immunochromatographic assay for the detection of norethindrone (NKET). Methods: A certain size colloidal gold solution was prepared by the method of trisodium citrate reduction. Anti-NKET monoclonal antibody was labeled. A certain concentration of coated antigen (NKET-OVA) and goat anti-mouse secondary IgG were sprayed on nitrocellulose Membrane, the preparation of colloidal gold immunochromatographic strip. Results: The test strip detection threshold of up to 500 ng / ml, the detection accuracy of 97.2%, the test strip stability and repeatability is good. Conclusion: The test strip has strong specificity, high sensitivity, quick and easy detection, no special equipment needed and great value in drug abuse screening.