紫外及红外吸收光谱是目前在有机化合物的结构研究中以及药物合成及鉴定等工作中,广泛应用的两种吸收光谱。其优点在于所需样品量少(1—2毫克),操作简便,费时间短(2—3小时),就能得出满意的结果。在我国这两种方法已开始普遍应用。本文拟对应用这两种光谱测定化合物结构时所经常遇到的一些问题做简短的介绍。紫外及紅外吸收光譜产生的一般原理分子吸收具有不同能量的光子后,变成分子振动的能量、转动的能量及电子跃迁所需要的能量,因此显示出三类光谱:振动光谱、转动光谱及电子光谱。因为转动能量较低, UV and IR absorption spectra are two kinds of absorption spectra which are widely used in the structure research of organic compounds and in the synthesis and identification of drugs. The advantage is that the required sample amount is small (1-2 mg), easy to operate, short time (2-3 hours), you can get satisfactory results. These two methods have started to be widely used in our country. This article intends to make brief introductions to some of the problems frequently encountered when using these two spectroscopic methods to determine the structure of a compound. General Principles of UV and IR Absorption Spectroscopy After absorbing photons of different energies into molecular vibrational energy, rotational energy, and energy required for electronic transitions, molecules exhibit three types of spectra: vibrational spectra, rotational spectra, and electrons spectrum. Because of low rotational energy,