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The methyl ester terminated G1 5,2 5 and 3 5 poly(amidoamine) dendrimers (PAMAM) are hydrolyzed by using NaOH to obtain external carboxylate groups with them (PAMAMC).These dendrimers show interaction with cytochrome C in some extent.For example,the reduction rate of Fe Ⅲ cytochrome C to Fe Ⅱ cytochrome C by ascorbic acid was clearly slowed down because of the interaction between cytochrome C and PAMAM dendrimers.The reaction happened only betweem Fe Ⅲ cytochrome C and ascorbic acid,it showed a pseudo first order constant of 3 48 min -1 .When PAMAMC (G2 5 or 3 5) dendrimers were added in the reaction system,the pseudo first order constant dropped to 1 40 or 1 32 ?min -1 .The association constant of 1:1 cytochrome C G2 5C is 2 4×10 4 and the association constant of 1:1 cytochrome C G3.5C is 2 6×10 4.
The methyl ester terminated G1 5,2 5 and 3 5 poly (amidoamine) dendrimers (PAMAM) are hydrolyzed by using NaOH to obtain external carboxylate groups with them (PAMAMC). The dendrimers show interaction with cytochrome C in some extent. For example, the reduction rate of Fe III cytochrome C to Fe II cytochrome C by ascorbic acid was clearly slowed down due to the interaction between cytochrome C and PAMAM dendrimers. The reaction happened only betweem Fe III cytochrome C and ascorbic acid, it showed a pseudo first order constant of 3 48 min -1 .When PAMAMC (G2 5 or 3 5) dendrimers were added in the reaction system, the pseudo first order constant dropped to 1 40 or 1 32 μ min -1. The association constant of 1: 1 cytochrome C G2 5C is 2 4 × 10 4 and the association constant of 1: 1 cytochrome C G3.5C is 2 6 × 10 4.