应用快速的ＤＮＡ抽提方法和现有的序标位（ＳＴＳ）引物，通过聚合酶链式反应（ＰＣＲ），检测了部分籼稻骨干亲本的多态性，建立了这些亲本的ＳＴＳ数据库。４２个标准路标ＳＴＳ中，有６个能在４０个亲本间揭示多态性，但各个ＳＴＳ揭示的多态性程度有差异；９个与目的性状连锁的ＳＴＳ中，有３个能揭示已知抗源与亲本间的多态性，有的ＳＴＳ在已知抗源扩增出的ＤＮＡ条带与所有亲本均不相同。数据库可为育种家制订实施标记辅助选择的育种计划提供依据。 The rapid DNA extraction method and the existing STS primers were used to detect the polymorphism of backbone parents of some Indica rice by polymerase chain reaction (PCR), and the STS databases of these parents were established. Sixty-two of the 42 standard signpost STSs revealed polymorphisms among 40 parents, but there was a difference in the degree of polymorphism revealed by each of the STSs; three of the nine STSs linked to the target trait revealed the known Anti-source and parent polymorphism, and some STS amplified DNA bands with known antigens are not the same with all the parents. The database may provide the basis for breeders to formulate breeding programs that implement marker-assisted selection.