论文部分内容阅读
拟检测林蛙皮多肽对大鼠皮肤激光损伤的修复作用及初步探索其作用途径。用激光照射大鼠背部皮肤,采用不同浓度林蛙皮多肽处理创面,观察其形态学变化,统计创面愈合率;检测Flg(参与表皮屏障功能建立的重要蛋白质之一)和Krt-14 mRNA(参与形成上皮细胞的细胞骨架)的差异。结果表明:第3、6天,低浓度(10 mg/m L)林蛙皮多肽作用组大鼠激光损伤皮肤愈合率显著高于阴性对照组(P<0.05),第6天,低浓度林蛙多肽作用组大鼠创伤皮肤中Flg的表达量是阴性对照组的1.908倍(P<0.000 1),Krt-14的表达量是阴性对照组的1.453倍(P<0.000 1)。这表明10 mg/m L林蛙皮多肽对大鼠激光损伤皮肤有修复作用,可能是通过促进损伤细胞中Flg和Krt-14 mRNA表达量的增加而发挥修复作用的。
To investigate the repair effect of Rana broomcorn polypeptide on laser cutaneous injury of rat skin and to explore its action pathway. Laser irradiation of the dorsal skin of rats was conducted. The morphological changes of the wounds were observed with different concentrations of Rana polygonum polypeptide, and the wound healing rate was calculated. Flg (one of the important proteins involved in the function of the epidermal barrier) and Krt-14 mRNA The cytoskeleton that forms epithelial cells). The results showed that the skin healing rate of laser-injured skin of rats treated with low-concentration (10 mg / m L) Rana bark skullcap polysaccharide was significantly higher than that of the negative control group on the 3rd and 6th days (P <0.05) The expression of Flg in traumatic skin of frog polypeptide-treated group was 1.908-fold (P <0.0001) compared with that of negative control group, and 1.453-fold (P <0.0001) in Krt-14 expression in negative control group. This indicates that 10 mg / m L Rana skin polypeptide has a repair effect on the laser-injured skin of rats, which may play a role in repair by increasing the expression of Flg and Krt-14 mRNA in injured cells.