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作者对179名婴儿分别于2、4、6月龄于大腿前外侧肌肉接种3剂乙型肝炎和DTP联合疫苗(DTP-HBV),并分别在接种前、第2次接种后2个月及第3次接种后1个月采血,检测抗体水平。由父母在日记卡上记录症状。本研究使用的疫苗由SmithKline公司生产,每剂0.5ml,含10μg HBsAg、≥30IU白喉类毒素、≥60IU破伤风类毒素和15OU百日咳杆菌。采用放射免疫法测定抗-HBs;用间接固相酶免疫法测定抗破伤风IgG和抗白喉IgG抗体滴度;用ELISA测定抗百日咳抗体滴度。
In this study, 179 infants were challenged with 3 doses of hepatitis B virus (DTP) and HBV combined vaccine (DTP-HBV) on the anterolateral thigh muscles at 2, 4, and 6 months of age respectively before vaccination, 2 months after the second vaccination and One month after the third vaccination, blood was collected and antibody levels were measured. Symptoms were recorded by parents on the diary card. Vaccines used in this study were manufactured by SmithKline at 0.5ml per dose containing 10μg HBsAg, ≥30IU diphtheria toxoid, ≥60IU tetanus toxoid and 15OU Bordetella pertussis. Anti-HBs were determined by radioimmunoassay. Titers of anti-tetanus IgG and anti-diphtheria IgG were measured by indirect enzyme-linked immunosorbent assay. The titers of anti-pertussis antibody were determined by ELISA.