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目的 :探讨人类肝癌细胞系 972 4在不同免疫力小鼠中的生长特点及T细胞对癌细胞的排斥特点。方法 :体外培养 972 4 ,接种到不同免疫力小鼠 (正常BALB/c ,B细胞缺陷的CBA/N ,T细胞缺陷的BALB/c nu ,T细胞、B细胞缺陷的SICD小鼠及免疫重建的SCID小鼠 )中 ,观察其生长特性 ;测定小鼠脾细胞杀伤力流式细胞仪测定外周血CD4 +,CD8+的百分率。结果 :BALB/c小鼠和免疫重建的BALB/c PBL SCID不成瘤 ;CBA/N随接种数量和途径不同而有不同的表现 ;BALB/c nu ,SCID小鼠和免疫重建的CBA/N PBL SCID10 0 %成瘤。接种过癌细胞的BALB/c和CBA/N小鼠脾细胞对癌细胞杀伤力较强 ,免疫重建的SCID杀伤力较小。不同组别CD4 +百分率都下降 ,CD8+变化不大 ,CD4 +/CD8+比值下降。结论 :小鼠成癌率与T细胞的作用最为密切 ;T细胞在异种瘤移植排斥中起主要的特异性的免疫杀伤作用
Objective : To investigate the growth characteristics of human hepatoma cell line 972 4 in different immunity mice and the characteristics of T cell rejection to cancer cells. METHODS: Cultured in vitro 972 4 and inoculated into different immune mice (normal BALB/c, B-cell deficient CBA/N, T-cell deficient BALB/c nu, T-cell, B-cell deficient SICD mice, and immune reconstitution The growth characteristics of the SCID mice were observed. The percentage of CD4 + and CD8 + in the peripheral blood was determined by flow cytometry. Results: BALB/c mice and immune reconstituted BALB/c PBL SCID were not tumorigenic; CBA/N had different manifestations depending on the number and route of inoculation; BALB/c nu, SCID mice and immune reconstituted CBA/N PBL SCID 10% tumor formation. The spleen cells of BALB/c and CBA/N mice that had been inoculated with cancer cells had a greater lethality to cancer cells, and the SCID of immune reconstitution was smaller. The percentage of CD4 + in the different groups decreased, CD8+ did not change significantly, and the ratio of CD4 +/CD8+ decreased. Conclusion :The tumorigenesis rate of mice is most closely related to T cells; T cells play a major role in the specific immune killing of xenograft rejection.