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目的:检测基质Cav-1(caveolin-1)、M2型丙酮酸激酶(pyruvate kinase M2,PKM2)和左旋乳酸脱氢酶B(L-lactate dehydrogenase B,LDH-B)在人肺腺癌(pulmonary adenocarcinoma,PAC)组织中的表达,探讨基质Cav-1对PAC侵袭和转移的影响。方法:利用量子点免疫荧光组织化学技术检测68例PAC组织和16例非癌变肺组织中基质Cav-1、LDH-B和PKM2的表达,同时应用量子点免疫荧光双标法检测基质Cav-1和α-平滑肌肌动蛋白(α-smooth muscle actin,α-SMA)的共表达。结果:基质Cav-1、LDH-B和PKM2在PAC组织中的阳性表达率分别为58.8%、54.4%和35.3%,在非癌变肺组织中的阳性表达率分别为100.0%、12.5%和6.3%,差异有统计学意义(P<0.05)。Cav-1、LDH-B和PKM2蛋白的表达与PAC患者的临床病理参数无相关性(P>0.05)。基质Cav-1与LDH-B和PKM2蛋白的表达呈负相关(P<0.05,r_s=-0.406;P<0.05,r_s=-0.320),基质LDH-B与PKM2蛋白的表达之间呈正相关(P<0.05,r_s=0.367)。结论:基质Cav-1表达缺失可能促进PAC的形成,其机制可能与上调LDH-B和PKM2的表达有关。
Objective: To detect the expression of caveolin-1, pyruvate kinase M2 (PKM2) and L-lactate dehydrogenase B (LDH-B) in human lung adenocarcinoma adenocarcinoma, PAC) and explore the effect of matrix Cav-1 on the invasion and metastasis of PAC. METHODS: The expression of stromal Cav-1, LDH-B and PKM2 in 68 cases of PAC and 16 cases of non-cancerous lung tissues were detected by quantum dot immunofluorescence histochemistry. Meanwhile, the expression of stromal Cav-1 And α-smooth muscle actin (α-SMA) co-expression. Results: The positive expression rates of Cav-1, LDH-B and PKM2 were 58.8%, 54.4% and 35.3% respectively in non-cancerous lung tissues, and the positive rates were 100.0%, 12.5% and 6.3% respectively %, The difference was statistically significant (P <0.05). The expression of Cav-1, LDH-B and PKM2 protein had no correlation with clinicopathological parameters of PAC patients (P> 0.05). The expression of stromal Cav-1 was negatively correlated with LDH-B and PKM2 (P <0.05, r_s = -0.406; P <0.05, r_s = -0.320) P <0.05, r_s = 0.367). CONCLUSION: The loss of stromal Cav-1 expression may promote the formation of PAC. Its mechanism may be related to the up-regulation of the expression of LDH-B and PKM2.