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目的:探讨间充质干细胞成血管因子受体表达特征及其意义。方法:采用经典的全骨髓贴壁法培养骨髓源间充质干细胞(MSC),通过成骨、成脂肪等多向诱导分化以及流式细胞分析其表面标记(CD34、CD90、CD29)等鉴定MSC特征;以P1-P17MSC为实验材料,利用RT-PCR的方法检测PDGFR、VEGFR和NRP1的表达。利用Transwell体外迁移体系初步探索VEGF在MSC迁移中的作用,同时利用MTT法评价VEGF对MSC增值的作用。结果:培养的MSC呈现出CD90、CD105强阳性,具有成骨、成脂肪等多向分化能力;强表达PDGFR-β和NRP1、不表达VEGFR(Flk1和Flt1)和PDGFR-α,VEGF促进了MSC的增殖和迁移。结论:VEGF可能通过PDGFR-NRP1促进了MSC的增值和迁移。
Objective: To investigate the expression and significance of mesenchymal stem cells expressing vascular receptors. Methods: Bone marrow-derived mesenchymal stem cells (MSC) were cultured by classical whole bone marrow adherent method. MSCs were identified by multi-directional induction of osteogenic and adipogenic differentiation and surface markers (CD34, CD90, CD29) The characteristics of PDGFR, VEGFR and NRP1 were detected by RT-PCR using P1-P17MSC as experimental material. Transwell vitro migration system was used to explore the role of VEGF in the migration of MSC. At the same time, MTT assay was used to evaluate the effect of VEGF on MSC proliferation. Results: The cultured MSCs showed strong positive of CD90 and CD105 with multi-directional differentiation ability such as osteogenesis and adiposity. Strong expression of PDGFR-β and NRP1, not express of VEGFR (Flk1 and Flt1) and PDGFR-α, Proliferation and migration. Conclusion: VEGF may promote the proliferation and migration of MSC through PDGFR-NRP1.