目的肺瘤平膏对共培养条件下人肺腺癌细胞(A549细胞)尿激酶类纤溶酶(u PA)、基质金属蛋白酶-2(MMP-2)、MMP-9 mRNA及蛋白表达的影响。方法制备肺瘤平膏含药血清,建立A549细胞与巨噬细胞的共培养体系,通过荧光定量PCR、Western blot及ELISA法观察肺瘤平膏对共培养条件下u PA、MMP-2、MMP-9 mRNA及蛋白表达的影响。结果共培养条件下,A549细胞变成间充质细胞样,而加入肺瘤平膏含药血清组,部分A549细胞形态恢复上皮细胞样。共培养条件下A549细胞中MMP-2、MMP-9及u PA蛋白的编码基因PLAU的mRNA表达量显著升高(P<0.05),肺瘤平膏含药血清干预后,其表达量均显著降低(P<0.05);共培养条件下A549细胞中MMP-2、MMP-9蛋白表达量显著升高(P<0.05),肺瘤平膏含药血清干预后,其表达量均显著降低(P<0.05);共培养上清液中MMP-2、MMP-9、u PA蛋白浓度均随时间延长逐渐升高,肺瘤平膏可以降低24 h后上清液中u PA的浓度(P<0.05),而对MMP-2、MMP-9的浓度无明显影响(P>0.05)。结论肺瘤平膏可以降低共培养条件下A549细胞中MMP-2和MMP-9的含量及上清液中u PA的含量,但不能降低上清液中MMP-2和MMP-9的含量。 Objective To investigate the effect of Huanruipingping on mRNA and protein expression of uPA, MMP-2 and MMP-9 in human lung adenocarcinoma A549 cells under co-culture condition . Methods The drug-containing serum of Pneumocystis preparations was prepared, and the co-culture system of A549 cells and macrophages was established. Fluorescence quantitative PCR, Western blot and ELISA were used to observe the effect of Pneumoconiosis on u PA, MMP-2, MMP -9 mRNA and protein expression. Results Under the co-culture conditions, A549 cells became mesenchymal-like, and the drug-containing serum was added to Hirschmann-ointment. Some A549 cells recovered epithelial-like cells. The co-cultured A549 cells in the MMP-2, MMP-9 and u PA protein encoding gene PLAU mRNA expression was significantly increased (P <0.05), Lung Lung Ping cream containing serum intervention, the expression was significant (P <0.05). The expression of MMP-2 and MMP-9 in A549 cells was significantly increased after co-culture (P <0.05). The expression of MMP-2 and MMP- P <0.05). The concentrations of MMP-2, MMP-9 and uPA in the co-culture supernatant all increased with time prolonging, <0.05), but had no significant effect on the concentration of MMP-2 and MMP-9 (P> 0.05). Conclusions Feiyunpingtong can reduce the content of MMP-2 and MMP-9 in A549 cells and the content of u PA in supernatant under co-culture condition, but can not reduce the content of MMP-2 and MMP-9 in supernatant.