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目的探讨大鼠单侧睾丸不同时间扭转、复位对双侧睾丸生殖细胞的影响以及可能机制。方法选用健康雄性SD大鼠35只,随机分成假手术组、单纯扭转组及扭转复位组。单纯扭转组采用Turner方法建立左侧睾丸扭转模型(左侧睾丸逆时针扭转720°),分别在扭转持续2、61、2h后即处死;扭转复位组在左侧睾丸扭转后26、1、2h再复位,复位后24h处死。留取双侧睾丸标本进行HE染色观察、生殖细胞凋亡检测及丙二醛(MDA)含量测定。结果睾丸扭转2h,单纯扭转及扭转复位后与假手术组比较扭转侧生殖细胞凋亡增加(P<0.05),而对侧生殖细胞凋亡增加差异无统计学意义(P>0.05);双侧MDA含量均增加(P<0.05)。随着扭转时间的延长,单纯扭转及扭转复位后细胞凋亡和MDA含量均明显增加(P<0.05)。结论随着扭转时间的延长,扭转复位后睾丸的损伤加重,其中缺血-再灌注的氧自由基损伤是造成睾丸损伤的可能机制之一。
Objective To investigate the effect of unilateral testicular torsion and resetting on testis germ cells at different times and its possible mechanism. Methods Thirty-five healthy male Sprague-Dawley rats were randomly divided into sham-operated group, untreated group and torsion-reduction group. Turbin method was used to establish the left testicular torsion model in the pure torsion group (the left testicle was rotated 720 ° counterclockwise), and the testicular torsion was continued for 2,61,2 h after torsion. The torsion reduction group was operated at 26,1,2 h Reset, reset after 24h died. Bilateral testicular specimens were collected for HE staining, germ cell apoptosis detection and malondialdehyde (MDA) content determination. Results The testicular torsion 2h, pure torsion and torsion reduction compared with the sham operation group increased germ cell apoptosis (P <0.05), while the contralateral germ cell apoptosis increased no significant difference (P> 0.05); bilateral MDA content increased (P <0.05). With the prolongation of torsion time, the apoptosis and the content of MDA increased after purely torsion and torsion reduction (P <0.05). Conclusion With the extension of the torsion time, the damage of testis after torsion reduction is aggravated. One of the possible mechanisms of testicular injury is ischemia-reperfusion injury of oxygen free radicals.