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目的通过测定苦碟子注射液中氨基酸的含量,为苦碟子注射液的质量评价提供依据。方法利用高效液相色谱法对其中的脯氨酸、缬氨酸、丙氨酸、苯丙氨酸、亮氨酸、异亮氨酸进行含量测定。结果苦碟子注射液中,脯氨酸浓度为53.04μg/ml;丙氨酸浓度为9.950μg/ml;缬氨酸浓度为6.913μg/ml;异亮氨酸浓度为4.065μg/ml;亮氨酸浓度为3.265μg/ml;苯丙氨酸浓度为2.631μg/ml。结论高效液相色谱法对苦碟子注射液中氨基酸的含量测定,测定方法简便准确,重现性好。
Objective To determine the content of amino acids in Kudiezi injection and provide the basis for the quality evaluation of Kudiezi injection. Methods The contents of proline, valine, alanine, phenylalanine, leucine and isoleucine were determined by high performance liquid chromatography. Results Kudzu injection, proline concentration of 53.04μg / ml; alanine concentration of 9.950μg / ml; valine concentration of 6.913μg / ml; isoleucine concentration of 4.065μg / ml; bright ammonia The acid concentration was 3.265 μg / ml; the phenylalanine concentration was 2.631 μg / ml. Conclusion HPLC method for the determination of the content of amino acids in Kudiezi injection, the determination method is simple and accurate, good reproducibility.