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目的:观察土贝母制剂对 SGC-7901人胃癌细胞的影响。方法:采用 MTT 分析法、流式细胞仪分析技术及透射电镜技术,体外实验观察土贝母诱导人胃癌细胞凋亡和分化。结果:当土贝母制剂浓度在5～200 μg/ml 范围内时,癌细胞抑制率呈浓度依赖性;浓度为20、50、80 μg/ml 时,分别对 SGC-7901细胞进行12 h 的作用,G_0加 G_1期细胞比率(与对照组比较)明显增加(P<0.05),S 期细胞比率明显减少(P<0.05),SGC-7901细胞可发生凋亡;20、50 μg/ml 作用12 h 时,在电镜下观察到典型的细胞凋亡的形态学改变,细胞核的变化最为突出。结论:土贝母制剂能阻止 G_0加 G_1期 SGC-7901细胞向 S 期进程,低浓度时可诱导人胃癌细胞凋亡。
Objective: To observe the effect of Fritillaria on SGC-7901 human gastric cancer cells. METHODS: MTT assay, flow cytometric analysis and transmission electron microscopy were used to observe the apoptosis and differentiation of gastric cancer cells induced by Fritillaria. Results: When the concentration of Fritillaria was in the range of 5-200 μg/ml, the inhibition rate of cancer cells was concentration-dependent; when the concentrations were 20, 50, and 80 μg/ml, SGC-7901 cells were treated for 12 h. Effect, G_0 and G_1 phase cell ratio (compared with the control group) increased significantly (P <0.05), S phase cell ratio was significantly reduced (P <0.05), SGC-7901 cells can undergo apoptosis; 20, 50 μ g / ml effect At 12 h, typical morphological changes of apoptosis were observed under the electron microscope, and the change of the nucleus was most prominent. Conclusion: Fritillaria can inhibit the progression of S phase in G_0 and G_1 SGC-7901 cells, and induce gastric cancer cell apoptosis in low concentration.