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目的 探讨肿瘤坏死因子 (TNF)、可溶性肿瘤坏死因子受体 (sTNFR)在胆囊结石致胆囊癌变的机理。方法 采用放射免疫法和双抗体夹心酶联免疫法对 5 3例胆囊结石和 9例胆囊癌伴结石病人血清及胆汁中TNF ,sTNFR水平进行检测。结果 胆囊结石及胆囊癌病人血清TNF水平均高于正常对照组 (P <0 0 5 ) ,且随着胆囊粘膜病变的加重而增高 ,在胆囊粘膜典型增生、不典型增生、胆囊癌 3组中 ,TNF逐级升高 ,每两组间均有显著性差异 (P <0 0 5 )。结论 TNF ,sTNFR参与了胆囊结石致癌的发病过程 ,且与胆囊癌的临床生物学特点密切相关。
Objective To investigate the mechanism of tumor necrosis factor (TNF) and soluble tumor necrosis factor receptor (sTNFR) in gallbladder carcinogenesis caused by gallstone. Methods Serum and bile TNF, sTNFR levels in 53 patients with gallstone and 9 patients with gallbladder cancer were measured by radioimmunoassay and double antibody sandwich enzyme-linked immunosorbent assay. Results The levels of serum TNF in gallbladder and gallbladder cancer patients were significantly higher than those in normal control group (P <0.05), and increased with the increase of gallbladder mucosa lesions. In the typical gallbladder mucosa hyperplasia, atypical hyperplasia and gallbladder carcinoma , TNF increased gradually, with significant difference between the two groups (P <0.05). Conclusion TNF and sTNFR are involved in the carcinogenesis of gallstone and are closely related to the clinical biological characteristics of gallbladder carcinoma.