在作者系列研究的基础上,本文报告应用单克隆抗体对恶性疟原虫(P.f)裂殖子表面的一种分子量20千道尔顿(Kd)的蛋白质进行了进一步的特性鉴定,并对其入侵红细胞的作用作了探讨。实验采用体外培养的恶性疟原虫冈比亚株(FCR-3),虫体经明胶山梨醇同步化后,根据实验要求标记~(35)(S)甲硫氨酸或~3[H]葡糖胺或~3[H]脯氨酸,然后继续培养至分析取 Based on a series of studies by the authors, the present paper reports the further characterization of a 20 kD protein of P.falciparum (Pf) merozoites using monoclonal antibodies and their identification The role of red blood cells were discussed. In this study, the P. falciparum Gambian strain (FCR-3) cultured in vitro was used. The symbionts were labeled with ~ (35) (S) methionine or ~ 3 [H] glucosamine after synchronization with gelatin sorbitol Or ~ 3 [H] proline, and then continue to culture until analysis