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目的探讨邻苯二甲酸二乙基己酯(DEHP)对青春期雄性大鼠睾丸及性激素的影响及潜在机制。方法 18日龄SD大鼠24只,按体重随机分为4组(对照组、低、中、高剂量DEHP组),每组6只。DEHP染毒剂量分别为250、500和750 mg/kg,经口连续染毒30 d;颈部脱臼处死,收集血清,分离睾丸组织;测定血清睾酮、黄体生成素(LH)和卵泡刺激素(FSH)等性激素水平及睾丸组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)、丙二醛和5α-还原酶(5α-R)活性。结果 DEHP处理组,大鼠睾丸和附睾脏器系数降低,睾丸曲精小管管腔缩小,结构坍塌,部分细胞膜破裂,细胞排列紊乱;与对照组比较,高剂量DEHP组大鼠血清睾酮和LH水平[分别为(115.04±14.21)pg/m L、(23.42±3.12)m IU/m L]降低,中、高剂量DEHP组大鼠血清FSH水平[分别为(5.49±0.42)、(4.63±0.51)IU/L]下降,差异有统计学意义(P<0.05);与对照组比较,高剂量DEHP组大鼠睾丸组织中GSH-Px和SOD活力[分别为(728.3±95.3)、(835.8±151.2)U/mg pro]降低,而中、高剂量DEHP组大鼠睾丸组织中丙二醛含量[分别为(79.6±13.9)、(90.6±4.0)nmol/mg pro]和5α-R2活性[分别为(415.9±112.7)、(415.9±147.8)IU/mg pro]升高,差异有统计学意义(P<0.05)。结论 DEHP暴露后,大鼠睾丸发生氧化应激,并通过诱导5α-R2活性降低血清睾酮水平。
Objective To investigate the effect of diethylhexyl phthalate (DEHP) on testis and sex hormone in adolescent male rats and its potential mechanism. Methods Twenty-four SD rats aged 18 days were randomly divided into 4 groups according to body weight (control group, low, medium and high dose DEHP group), 6 in each group. The DEHP doses were 250, 500 and 750 mg / kg, respectively, for 30 consecutive days. The rats were sacrificed by cervical dislocation and the serum was collected to separate the testicular tissue. The levels of serum testosterone, luteinizing hormone (LH) and follicle stimulating hormone FSH) and other sex hormones and testicular tissue glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), malondialdehyde and 5α-reductase (5α-R) activity. Results The organ coefficient of testis and epididymis in DEHP treatment group decreased, the testicular seminiferous tubules reduced in size, the structure collapsed, some of the cell membrane ruptured and the cells arranged in disorder. Compared with the control group, the levels of serum testosterone and LH in high dose DEHP group [(115.04 ± 14.21) pg / m L and (23.42 ± 3.12) m IU / m L], respectively. The levels of serum FSH in DEHP group were (5.49 ± 0.42) and (4.63 ± 0.51 (P <0.05). Compared with the control group, the activities of GSH-Px and SOD in testis tissue of high-dose DEHP group were (728.3 ± 95.3) and (835.8 ± 151.2) U / mg pro], while the content of malondialdehyde ([(79.6 ± 13.9), (90.6 ± 4.0) nmol / mg pro] and 5α-R2 activity in testicular tissue of rats in middle and high dose DEHP group [ Respectively (415.9 ± 112.7) and (415.9 ± 147.8) IU / mg pro], the difference was statistically significant (P <0.05). Conclusion After DEHP exposure, oxidative stress occurs in the testis of rats and serum testosterone level is decreased by inducing 5α-R2 activity.