利用高灵敏度、全自动化的方法对微量和痕量样本的DNA分型一直是法医学工作的重点与难点。焦磷酸测序技术具有实时监测、高通量、无需胶检测和荧光标记、定量性能好、结果准确和可自动化的优势。本文就焦磷酸测序化学反应测序的原理、流程及其在法医学SNP分型、建立标准化分型体系、大规模人群数据库建设及与其他常用分型技术的比较做一综述。 It has always been the focus and difficulty of forensic work to use high sensitivity and fully automated DNA typing methods to trace and trace samples. Pyrosequencing technology has the advantages of real-time monitoring, high throughput, no gel detection and fluorescence labeling, good quantitative performance, accurate results and automation. In this paper, pyrosequencing chemical sequencing of sequencing principles, processes and forensic SNP typing, the establishment of a standardized typing system, large-scale population database construction and comparison with other commonly used typing techniques are reviewed.