应用超薄层聚丙烯酰胺凝胶等电聚焦电泳对武汉地区198例成人精液黄递酶Ⅲ(DIA_3)作了分型。观察到4种表现型,基因频率分别为DIA_3~1=0.7727;DIA_3~2=0.2172;DIA_3~3=0.0101。对保存精斑进行检测证实,室温下保存9周的精斑仍能作DIA_3分型。在聚丙烯酰胺凝胶中引入分离剂HEPES,提高了分辨率。改进后的等电聚焦技术尤适于DIA_3基因产物的分离。文中还调查了我国汉族人群DIA_3频率分布,并与其它群体资料作了比较。 198 cases of adult semen diaphorase Ⅲ (DIA_3) in Wuhan area were classified by isoelectric focusing electrophoresis with ultrathin layer polyacrylamide gel. Four phenotypes were observed. The frequencies of genes were DIA_3 ~ 1 = 0.7727, DIA_3 ~ 2 = 0.2172, DIA_3 ~ 3 = 0.0101, respectively. Semen preserved for testing confirmed that preserved at room temperature for 9 weeks can still make DIA_3 type. Introduced in the polyacrylamide gel separating agent HEPES, increased resolution. The improved isoelectric focusing technology is especially suitable for the separation of DIA_3 gene products. The paper also investigated the frequency distribution of DIA_3 in Chinese Han population and compared with other population data.