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目的 :本文对超抗原葡萄球菌肠毒素B(SEB)活化的Th1细胞介导的对肿瘤细胞杀伤作用进行了研究。方法 :人外周血淋巴细胞与SEB共同培养 ,用流式细胞术测定增殖细胞亚群 ;用酶联双夹心法测定IL 2 ,IL 4水平 ;用K5 6 2 ,HL 6 0肿瘤细胞和自身淋巴细胞作为靶细胞测定效应细胞和细胞因子的杀伤活性。结果 :SEB共同培养 6d的淋巴细胞 ,CD4+T细胞由37.5 %增加到 48.5 % ;CD16 +淋巴细胞由 14.3%增加到 2 7.9% ;CD8+T细胞无明显增加。预先去除CD8+T细胞不影响SEB对CD4+T细胞的活化作用。结论 :SEB活化CD4+T细胞是Th1而不是Th2细胞。它们释放大量的IL 2而不是IL 4。活化的Th1细胞介导了效应细胞和细胞因子对肿瘤细胞的非特异性杀伤效应 ,但是不能介导CTL的杀伤作用
Objective: To study the killing effect of superantigen-activated Th1 cells from enterotoxin B (SEB) on tumor cells. METHODS: Human peripheral blood lymphocytes were co-cultured with SEB, proliferating cell subpopulations were determined by flow cytometry, IL 2 and IL 4 levels were determined by enzyme-linked double sandwich assay, and K562, HL 60 tumor cells and autologous lymphocytes were used. The cells serve as target cells to determine the killing activity of effector cells and cytokines. RESULTS: SEB co-cultured 6 days of lymphocytes, CD4+ T cells increased from 37.5 % to 48.5%; CD16+ lymphocytes increased from 14.3% to 27.9%; CD8+ T cells did not increase significantly. Removal of CD8+ T cells in advance did not affect the activation of CD4+ T cells by SEB. Conclusion: SEB activated CD4+ T cells are Th1 but not Th2 cells. They release large amounts of IL2 instead of IL4. Activated Th1 cells mediate non-specific killer effects of effector cells and cytokines on tumor cells, but do not mediate CTL killing.