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经Vero细胞培养的P3株乙脑灭活病毒，以超滤浓缩和硫酸色精蛋白处理后，40％蔗糖垫层在27500r／min离心6小时，收集沉淀制成提纯乙脑疫苗。其蛋白含量在0．012～0.035mg／ml，稀释8倍后其疫苗效价仍能超过日本提纯鼠脑乙脑疫苗参考标准和我国乙脑疫苗参考标准。Vero细胞残余DNA＜100pg／0．5ml，残余牛血清＜1μg／ml。
The Vero cells cultured P3 JE virus inactivated by ultrafiltration and protamine sulfate treatment, 40% sucrose cushion at 27500r / min for 6 hours, the precipitate was collected to prepare purified JE vaccine. The protein content of 0.012 ~ 0.035mg / ml, diluted eight times its vaccine titer can still exceed the Japanese Encephalitis B virus vaccine reference standard and our JE vaccine reference standard. Vero cell residual DNA <100 pg / 0.5 ml, residual bovine serum <1 μg / ml.