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采用柱色谱法对桑叶提取物中黄酮、生物碱、多糖组分进行富集分离;采用葡萄糖氧化酶法,利用蔗糖为底物,以α-葡萄糖苷酶抑制模型对桑叶多组分进行评价;采用等效线法、周-特氏联合指数法和等辐射分析法评价2组分间的药效相互作用及量效特点,为揭示桑叶降血糖的作用机制提供科学依据。成分分析表明,桑叶提取物中,黄酮质量分数为5.3%,有机酚酸质量分数为10.8%,1-脱氧野尻霉素(DNJ)质量分数为39.4%,多糖质量分数为18.9%。活性评价表明,桑叶黄酮、生物碱、多糖组分均具有显著的α-葡萄糖苷酶抑制活性,其抑制率随各组分浓度的增加而升高,3个组分中桑叶生物碱抑制活性最强。桑叶生物碱与桑叶黄酮配伍、桑叶生物碱与桑叶多糖配伍均表现出协同增效作用,但多糖与黄酮组配伍未见明显的协同作用,从而证实了桑叶多组分调节血糖的相互作用,为桑叶多组分降血糖物质基础与作用机制的揭示提供了科学依据。
The contents of flavonoids, alkaloids and polysaccharides in mulberry leaf extracts were separated by column chromatography. The multi-components of mulberry leaf were separated by glucose oxidase method and sucrose as substrate, and α-glucosidase inhibition model Evaluation; Equivalent line method, the week - combination of Tertull index method and the other radiation analysis of the evaluation of the two components of the pharmacodynamic interaction and the characteristics of quantum efficiency, to reveal the mechanism of mulberry leaf hypoglycemic provide a scientific basis. Composition analysis showed that the content of flavonoids in mulberry leaf extract was 5.3%, the content of organic phenolic acids was 10.8%, the content of 1-deoxynojirimycin (DNJ) was 39.4% and the content of polysaccharides was 18.9%. The activity evaluation showed that mulberry leaf flavonoids, alkaloids, polysaccharides have significant α-glucosidase inhibitory activity, the inhibition rate increased with the increase of the concentration of each component, the three components of mulberry alkaloids inhibited Most active. Mulberry leaf alkaloids and mulberry flavonoids compatibility, mulberry leaf alkaloids and mulberry leaf polysaccharide compatibility synergy, but polysaccharide and flavonoids group no significant synergies, thus confirming the mulberry leaf multi-component regulation of blood glucose , Which provided a scientific basis for revealing the basis and mechanism of hypoglycemic substances in mulberry leaves.