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目的探讨外源性酸性成纤维细胞生长因子(aFGF)对新生大鼠缺氧缺血性脑损伤血管新生的作用及可能机制。方法以7日龄SD大鼠制作HIBD的动物模型,应用免疫组织化学染色方法观察各组脑血管内皮细胞生长因子(VEGF)的表达及血管新生情况。结果免疫组化检测VEGF的表达,治疗组VEGF的表达从缺氧后第1天开始增加,到第3天时明显高于对照组(P<0.01),第7天3组之间无统计学差别。治疗组的微血管数目在缺氧缺血后第1天显著高于对照组(P<0.01),第3天还高于对照组(P<0.05),第7天仍高于假手术组(P<0.01)。结论aFGF使缺氧缺血大鼠脑组织VEGF表达增多,可能是促进脑组织新生血管形成的重要因素之一,提示早期应用外源性aFGF对于HIBD新生大鼠有一定的治疗作用。
Objective To investigate the effect and possible mechanism of exogenous acidic fibroblast growth factor (aFGF) on angiogenesis in neonatal rats with hypoxic-ischemic brain damage. Methods HIBD animal models were made on 7-day-old SD rats. The expression of vascular endothelial growth factor (VEGF) and angiogenesis were observed by immunohistochemical staining. Results The expression of VEGF was detected by immunohistochemistry. The expression of VEGF in the treatment group increased from the first day after hypoxia to the third day and significantly higher than that in the control group (P <0.01). There was no significant difference between the three groups on the seventh day . The number of microvessels in the treatment group was significantly higher than that in the control group on the first day after hypoxia-ischemia (P <0.01), higher on the third day than the control group (P <0.05) <0.01). CONCLUSIONS: aFGF increases the expression of VEGF in brain tissue of hypoxic-ischemic rats, which may be one of the important factors that promote neovascularization in brain tissue. It suggests that early administration of exogenous aFGF may have some therapeutic effects on neonatal HIBD rats.