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目的:从杉叶厥藻中提取多糖类活性物质,并测定其抗病毒活性。方法:水提醇沉法提取粗多糖,以活性为导向,DEAE-Cellulose离子交换层析和Sephadex G-200凝胶柱层析法进行精制。制备的多糖样品测定理化性质,并进行纳米硒化,MTT法测定细胞毒性,CPE法测定抗柯萨奇病毒B3活性。结果:杉叶厥藻粗多糖属硫酸多糖,其多糖平均含量为27.9%,硫酸基平均含量为11.5%;粗多糖纯化得率为66.3%;多糖经纳米硒化后,平均粒径为28.6nm;纯多糖SCp11的半数有效浓度为2.2 mg/mL,治疗指数为3057.0;多糖硒化后,细胞毒性略有增加,但活性增强,治疗指数增加。结论:杉叶厥藻多糖及其硒化产物是一种有开发前景的抗病毒活性物质。
OBJECTIVE: To extract the polysaccharide active substance from A. vulgaris and determine its antiviral activity. Methods: The crude polysaccharides were extracted by water extraction and alcohol precipitation, and their activities were guided. The purification was performed by DEAE-Cellulose ion exchange chromatography and Sephadex G-200 gel column chromatography. The prepared polysaccharide samples were measured for physicochemical properties, and nano-selenium was used. The cytotoxicity was measured by MTT assay, and the anti-Coxsackievirus B3 activity was measured by CPE method. Results: The crude polysaccharide of A. sauteri was a sulfated polysaccharide with an average polysaccharide content of 27.9% and an average sulfate content of 11.5%. The crude polysaccharide was purified at a rate of 66.3%. The polysaccharide was nanoselenized and had an average particle size of 28.6 nm. The effective concentration of polysaccharide SCp11 was 2.2 mg/mL, and the therapeutic index was 3057.0. After the polysaccharide was selenized, the cytotoxicity was slightly increased, but the activity was enhanced and the therapeutic index was increased. Conclusion: The polysaccharides and their selenization products of A. fruticosa are a promising antiviral active substance.