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以构树幼苗根组织和叶组织为试材,测定不同浓度NaCl胁迫下液泡膜H+-ATPase的活性、液泡膜脂肪酸组成和膜的流动性。结果表明:在低浓度NaCl胁迫下,根组织中液泡膜H+-ATPase的活性有所增加,当胁迫浓度为150mmol·L-1时,活性又有所下降,但接近对照水平。而叶组织液泡膜H+-ATPase的活性在100mmol·L-1时降低,高浓度NaCl(150mmol·L-1)处理后又有所升高。液泡膜脂肪酸基本组成为C16:0,C16:1,C18:0,C18:1,C18:2和C20:0。盐胁迫后构树幼苗液泡膜脂肪酸组分相对含量发生变化。根组织液泡膜脂肪酸不饱和度下降,150mmol·L-1NaCl处理后有所升高;而在叶组织中,液泡膜脂肪酸不饱和度在50mmol·L-1NaCl处理后下降,随着盐胁迫浓度的增加,不饱和度又高于对照。NaCl胁迫下,构树液泡膜流动性和脂肪酸不饱和度的变化趋势相一致。脂肪酸不饱和度下降时,膜流动性下降,反之则膜流动性上升。研究结果为深入了解木本植物液泡膜H+-ATPase活性、脂肪酸组成和膜的流动性在盐胁迫下的适应及其3者的相互关系提供了参考。
The root and leaf tissue of the bark tree seedlings were used as test materials to measure the activity of tonoplast H + -ATPase, the composition of tonoplast fatty acid and the fluidity of membrane under different concentrations of NaCl stress. The results showed that under low NaCl stress, the activity of tonoplast H + -ATPase increased in root tissues. When the stress concentration was 150 mmol·L-1, the activity dropped to the level of control. However, the activity of vacuolar membrane H + -ATPase decreased in 100 mmol·L-1 and increased after treated with high concentration of NaCl (150 mmol·L-1). The basic components of tonoplast fatty acids are C16: 0, C16: 1, C18: 0, C18: 1, C18: 2 and C20: 0. Changes in relative content of tonoplast fatty acid components of tree seedlings under salt stress. In the leaf tissue, the degree of unsaturation of tonoplast fatty acids decreased after treatment with 50 mmol·L-1 NaCl, but decreased with the increase of salt stress concentration Increase, the degree of unsaturation is higher than the control. Under NaCl stress, the change trend of vacuolar membrane fluidity and fatty acid unsaturation was the same. When fatty acid unsaturation declines, membrane fluidity decreases, whereas membrane fluidity increases. The results provide a reference for further understanding of H + -ATPase activity, fatty acid composition and membrane fluidity of tonoplast in woody plants under salt stress and their interrelations.