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以华黄芪叶片为外植体，在附加不同浓度和比例的２，４－Ｄ与６－ＢＡ的ＭＳ培养基上，探讨植物生长调节物质对华黄芪愈伤组织诱导、增殖及芽分化的效应，并在愈伤组织增殖过程中，初步对愈伤指数与愈伤组织生长量的关系进行了比较，结果表明：在ＭＳ＋２，４－Ｄ０．５ｍｇ／Ｌ＋６－ＢＡ０．３ｍｇ／Ｌ培养基上诱导率最高，且形成愈伤组织时间最短；在ＭＳ＋２，４－Ｄ０ｍｇ／Ｌ＋６－ＢＡ０．３ｍｇ／Ｌ培养基上，芽的分化率最高；愈伤指数与“称重法”测定愈伤组织生长所得结果一致．
Using Astragalus membranaceus as explants, the effects of plant growth regulators on induction, proliferation and bud differentiation of Astragalus callus were studied on MS medium supplemented with 2,4-D and 6-BA at different concentrations and ratios , And the relationship between callus index and callus growth was preliminary compared during the process of callus proliferation. The results showed that the callus induction was induced on MS + 2,4-D0.5mg / L + 6-BA0.3mg / L medium And the callus formation time was the shortest. The differentiation rate of shoots was the highest in MS + 2,4-D0mg / L + 6-BA0.3mg / L medium, and the callus index and “weight method” The result is the same.