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目的采用荧光原位杂交技术(fluorescence in situ hybridization,FISH)检测宫颈病变细胞端粒酶的表达,探讨端粒酶活性与宫颈癌发生发展的相关性。方法选择CINI108例,CINⅡ50例,CINⅢ35例,宫颈鳞癌38例,腺癌14例,正常对照组40例,年龄22～65岁,平均年龄44.2岁。采用FISH技术检测TERC基因,分析各级宫颈病变组织中hTERC基因的扩增情况,同时与HPV(SPR法)检测结果作比较,观察其检测效果。结果随宫颈病变级别的加重,hTERC基因阳性扩增率呈逐渐增高趋势。组间有显著统计学差异(χ2=158.395,P=0.000)。其检测宫颈上皮瘤变和宫颈癌的敏感性及特异性均明显优于HPV。结论端粒酶作为一种预测性标记物,有望成为宫颈癌早期筛查方法。
Objective To detect the expression of telomerase in cervical lesions by fluorescence in situ hybridization (FISH), and to explore the correlation between telomerase activity and the occurrence and development of cervical cancer. Methods CINI 108 cases, CIN Ⅱ 50 cases, CIN Ⅲ 35 cases, cervical squamous cell carcinoma 38 cases, adenocarcinoma 14 cases, normal control group 40 cases, aged 22 to 65 years, mean age 44.2 years. TERC gene was detected by FISH, and the amplification of hTERC gene in cervical lesions at all levels was analyzed. Meanwhile, the detection results were compared with that of HPV (SPR method). Results With the severity of cervical lesions, the positive rate of hTERC gene amplification increased gradually. Significant statistical differences between groups (χ2 = 158.395, P = 0.000). The detection of cervical epithelial tumor and cervical cancer sensitivity and specificity were significantly better than HPV. Conclusion Telomerase as a predictive marker is expected to be an early screening method for cervical cancer.