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建立了５种喹诺酮类药物的毛细管胶束电动色谱分离和尿样品的定量测定方法。测定条件为：运行缓冲液：５０ｍｍｏｌ／Ｌ磷酸二氢钠－１００ｍｍｏｌ／Ｌ溴化十六烷基三甲胺（１∶１，ｐＨ４．５）；操作电压１２ＫＶ，检测波长２８０ｎｍ。２０ｍｉｎ内５种喹诺酮药物全部得到分离，并与毛细管区带电泳作了比较。尿液对样品的测定无干扰。线性范围为５．０～３５．０ｍｇ／Ｌ，最低检测浓度为２ｍｇ／Ｌ，ＲＳＤ小于１０％。
A method for the quantitative determination of five quinolones by capillary micellar electrokinetic chromatography and urine samples was established. The measurement conditions were as follows: running buffer: 50 mmol / L sodium dihydrogen phosphate - 100 mmol / L cetyltrimethylammonium bromide (1: 1, pH 4.5); operating voltage 12 kV and detection wavelength 280 nm. All five quinolone drugs were separated within 20 min and compared with capillary zone electrophoresis. Urine no interference on the determination of the sample. The linear range of 5.0 ~ 35.0mg / L, the lowest detection concentration of 2mg / L, RSD less than 10%.