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目的:建立红药片的HPLC含量测定方法。方法:色谱柱:Agilent Extend-C_(18)(250 mm×4.6 mm,5μm),流动相:乙腈-0.3%磷酸溶液(19:81),流速:1.0ml·min~(-1),检测波长:203 nm,柱温:40℃。结果:三七皂苷R_1在0.03～0.90μg范围内线性关系良好(r=0.999 9);平均加样回收率为100.0%,RSD=1.3%(n=5);人参皂苷Rg_1在0.10～3.00μg范围内线性关系良好(r=1.000 0);平均加样回收率为99.5%,RSD=0.6%(n=5)。结论:该方法简便易行,结果准确,可适用于红药片的质量控制。
Objective: To establish a HPLC method for determination of red tablets. Method: Column: Agilent Extend-C_(18)(250 mm×4.6 mm, 5 μm), mobile phase: acetonitrile-0.3% phosphoric acid solution (19:81), flow rate: 1.0 ml·min -1, detection Wavelength: 203 nm, column temperature: 40°C. Results: The linear relationship of notoginsenoside R 1 in the range of 0.03-0.90 μg was good (r=0.999 9); the average recovery was 100.0%, RSD was 1.3% (n=5), and the ginsenoside Rg_1 was 0.10-3.00 μg. The linearity in the range was good (r=1.000 0); the average recovery was 99.5%, and the RSD was 0.6% (n=5). Conclusion: This method is simple, accurate, and suitable for quality control of red pills.