本文报道经临床及染色体分析证实的４７例Ｋｌｉｎｅｆｅｌｔｅｒ综合征患者，其中３６例连续使用睾酮治疗１年以上，青春发育顺序以阴毛生长最快，其次为腋毛和胡须，最后为喉节。阴茎长度从５．２６ｃｍ增长到８．２３ｃｍ，睾丸容积右侧从３．１ｍｌ增大到４．８ｍｌ，左侧从３．０９ｍｌ到４．７０ｍｌ，精液量从０．５～１ｍｌ增多到１．５～４．４ｍｌ，说明睾丸酮有促发附属性腺分泌功能。但患者仍不育。睾丸酮治疗３月后患者性功能即有明显改善。 In this paper, 47 cases of Klinefelter syndrome confirmed by clinical and chromosomal analysis were reported. Among them, 36 cases were treated with testosterone continuously for more than 1 year. The sequence of youth development was the pubic hair grows fastest, followed by the armpit hair and beard. The length of the penis increased from 5.26 cm to 8.23 ​​cm, the volume of testis increased from 3.1 ml to 4.8 ml on the right, 3.09 ml to 4.70 ml on the left, and semen increased from 0.5 to 1 ml. 5 ~ 4.4ml, indicating that testosterone promote appendage gland secretion. But the patient is still infertile. After 3 months of testosterone treatment of patients with significant improvement in sexual function.