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目的:探讨荧光探针SNFYMPLGGGSK-FITC与胃癌组织的结合能力,预测其在胃癌诊断中的应用价值。方法:收集我院2015年6月至10月收治的48例胃癌患者手术切除的肿瘤和癌旁组织,使用异硫氰酸荧光素FITC标记的线性七肽SNFYMPL及无关序列肽探针对其冰冻切片进行荧光染色,通过共聚焦激光扫描显微镜CLSM检测其结合能力,并与组织病理结果对比。结果:SNFYMPLGGGSK-FITC对胃癌组织荧光染色的阳性率为81.25%(39/48),明显优于无关序列肽探针的14.58%(7/48),差异有统计学意义(P<0.001)。SNFYMPLGGGSK-FITC对癌旁组织荧光染色的阳性率为27.08%(13/48),明显低于对胃癌组织荧光染色阳性率,差异有统计学意义(P<0.001)。结论:FITC标记的SNFYMPL短肽探针与胃癌组织较强结合能力,图像组织分辨率好,可以作为共聚焦激光显微内镜在胃癌诊断的潜在靶向分子进一步研究。
Objective: To investigate the binding ability of fluorescent probe SNFYMPLGGGSK-FITC to gastric cancer tissue and to predict its value in the diagnosis of gastric cancer. Methods: Forty-eight gastric cancer and para-cancerous tissues were collected from June 2015 to October 2015 in our hospital. FITC labeled linear heptapeptide SNFYMPL and unrelated sequence peptide probe were used to freeze The sections were stained with fluorescence, and their binding ability was detected by Confocal Laser Scanning Microscope (CLSM), and compared with the histopathological results. Results: The positive rate of SNFYMPLGGGSK-FITC staining was 81.25% (39/48), which was significantly better than 14.58% (7/48) of unrelated peptide probes. The difference was statistically significant (P <0.001). The positive rate of SNFYMPLGGGSK-FITC fluorescence staining of adjacent tissues was 27.08% (13/48), which was significantly lower than that of the positive staining of gastric cancer (P <0.001). CONCLUSION: The FITC-labeled SNFYMPL short peptide probe has strong binding ability with gastric cancer tissue and good resolution of image tissue. It can be used as a potential target molecule for the diagnosis of gastric cancer by confocal laser micro-endoscopy.