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将常规体外培养的恶性疟原虫经5%山梨醇同步处理后,与阳离子荧光染料若丹明123,于37℃孵育30分钟,在4℃下用PBS洗涤3次后,继续培养,以观察染料对疟原虫生长的作用。结果表明若丹明抑制疟原虫生长的浓度≥5μM,50%有效抑制浓度为6μM。疟原虫奋发育期对染料的敏感性不一,环状体和滋养体经染料处理后形成的新环状体大大减少,而处理过的裂殖体所形成的新环状体比未经处理的减少不多,说明环状体和滋养体较裂殖体为敏感。如果感染的红细胞预
Conventional in vitro cultured P. falciparum was treated with 5% sorbitol, incubated with rhodamine 123, a cationic fluorescent dye, at 37 ° C for 30 minutes and washed 3 times with PBS at 4 ° C. Effect on Plasmodium growth. The results showed that rhodamine inhibited Plasmodium growth at a concentration of 5 μM and a 50% effective inhibitory concentration of 6 μM. Plasmodium had different sensitivities to dyes during development. The number of rings and trophozoites that were treated by dye treatment decreased greatly, whereas the newly formed ring formed by the treated schizonts was significantly less than that of untreated Less reduction, indicating that the ring and trophozoites than schizonts for sensitive. If infected red blood cells pre-