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家兔从妊娠第1天始,每天经口给予炔诺酮肟4mg/kg,连续3天,第9天处死。取卵巢作HE、ACP、ALP、SDH及3β-HSD染色,部分卵巢作超微结构观察。结果表明:炔诺酮肟呈明显的抗着床作用,卵巢黄体平坦,缩小。光镜观察,黄体细胞呈较明显的颗粒变性及空泡变性。组化染色,ACP活性增强(P<0.01)。3β-HSD活性减弱(P<0.01),ALP及SDH活性无明变化(P>0.05)。超微结构可见,黄体细胞胞质中滑面内质网明显减少,脂滴聚集,溶酶体数量增多,形状多样,脂质空泡化及类似髓鞘样结构等。提示由于药物抑制了卵巢3β-HSD活性,使卵巢激素生物合成受阻;此外,ACP活性增加,提示溶酶体通透性增加。
Rabbits from the first day of pregnancy, oral administration of norethisterone oxime 4mg / kg daily for 3 days, the first 9 days of death. Ovaries were taken for HE, ACP, ALP, SDH and 3β-HSD staining. Some ovaries were observed by ultrastructure. The results showed that norethisterone oxime showed a significant anti-implantation effect and ovarian corpus luteum was flat and contracted. Light microscopic observation, corpus luteum cells were more obvious degeneration and vacuolar degeneration. Histochemical staining and ACP activity increased (P <0.01). 3β-HSD activity decreased (P <0.01), ALP and SDH activity had no change (P> 0.05). The ultrastructure shows that the smooth endoplasmic reticulum in the cytoplasm of luteal cells is obviously reduced, the lipid droplets gather, the number of lysosomes increases, the shapes are diverse, the lipid vacuoles and similar myelin-like structures. It is suggested that the inhibition of ovarian 3β-HSD activity inhibits ovarian hormone biosynthesis. In addition, the activity of ACP is increased, suggesting an increase in lysosomal permeability.