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目的检测不同浓度褪黑素对体外培养的人骨肉瘤细胞系MG-63细胞增殖的影响,为更好地应用褪黑素辅助治疗骨科疾病提供一定的实验基础。方法在体外培养的MG-63细胞中,外源性加入1 nmol/L~10 mmol/L浓度褪黑素,用CCK-8的方法检测不同浓度褪黑素对细胞增殖的影响,用PI染色流式细胞术检测其对细胞周期分布的影响。结果 CCK-8分析发现4~10mmol/L浓度的褪黑素可以以一种时间依赖性和剂量依赖性的方式显著抑制MG-63细胞的增殖,而1 nmol/L~2 mmol/L浓度的褪黑素对MG-63细胞的增殖没有显著影响。无论将MG-63细胞周期同步还是非同步,流式细胞仪的检测显示,4~10 mmol/L浓度褪黑素可以显著增加细胞周期G0/G1期的比例,同时减少S和G2/M期的比例;而1 nmol/L~2 mmol/L浓度褪黑素对MG-63细胞周期的分布没有显著影响。结论褪黑素可以以一种时间依赖性和剂量依赖性方式抑制骨肉瘤MG-63细胞的增殖,这种抑制效应与细胞周期分布的改变和G0/G1期停滞有关。
Objective To investigate the effects of different concentrations of melatonin on the proliferation of human osteosarcoma cell line MG-63 in vitro and to provide some experimental basis for the better application of melatonin in the treatment of orthopedic diseases. Methods Melatonin (1 nmol / L ~ 10 mmol / L) was exogenously added into MG-63 cells cultured in vitro. The effects of different concentrations of melatonin on proliferation were detected by CCK-8. PI staining Flow cytometry was used to examine the effect on cell cycle distribution. Results CCK-8 analysis showed that melatonin concentration of 4 ~ 10mmol / L significantly inhibited the proliferation of MG-63 cells in a time-dependent and dose-dependent manner, while the concentration of 1nmol / L ~ 2mmol / L Melatonin had no significant effect on the proliferation of MG-63 cells. Flow cytometry showed that melatonin at a concentration of 4-10 mmol / L significantly increased the proportion of cells in G0 / G1 phase and decreased both S and G2 / M phase, regardless of the cell cycle synchronization or asynchrony. While the concentration of melatonin of 1 nmol / L ~ 2 mmol / L had no significant effect on the cell cycle distribution of MG-63 cells. Conclusion Melatonin can inhibit the proliferation of osteosarcoma MG-63 cells in a time-dependent and dose-dependent manner. The inhibitory effect is related to the change of cell cycle distribution and the arrest of G0 / G1 phase.