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目的:观察参附注射液对肺缺血再灌注损的保护作用,并探讨其机制。方法:将30只兔随机分为假手术组(Sh组)、肺缺血再灌注组(IR组)和参附注射液组(SF组),每组10只,建立肺IR模型。Sh组于左肺门处以弯眼科镊引导7号指示线从下方绕过肺门,不予钳夹,于6h后取肺组织;IR组于留置指示线外侧用无创伤微血管钳钳夹肺门远心脏一端,阻断肺门45min,再开放6h后取肺组织;SF组于阻断肺门前30min耳缘静脉注射SF10mL/kg。采用流式细胞术检测肺组织细胞凋亡率,采用原位杂交检测肺组织Bcl-2/Bax、Fas/FasL基因表达。结果:与Sh组比较,IR组、SF组肺组织细胞凋亡率及Bcl-2/Bax、Fas/FasL基因表达明显增加(P<0.01);与IR组比较,SF组肺组织细胞凋亡率及Bcl-2/Bax、Fas/FasL基因表达明显降低(P<0.05)。结论:参附注射液对肺缺血再灌注损具有保护作用,与其抑制肺组织肺组织Bcl-2/Bax、Fas/FasL基因表达、减少细胞凋亡有关。
Objective: To observe the protective effect of Shenfu injection on lung ischemia-reperfusion injury and to explore its mechanism. Methods: Thirty rabbits were randomly divided into sham operation group (Sh group), lung ischemia-reperfusion group (IR group) and Shenfu injection group (SF group), with 10 rats in each group. Sh group in the left hilar Department of eye tweezers guide line No. 7 around the hilar from below, not clamp, take lung tissue after 6h; IR group indwelling indication line with non-invasive microvascular forceps clamp Hilar Far end of the heart, hilar block 45min, and then open lung tissue after 6h; SF group in the front of the hilar block 30min before injection of SF10mL / kg. The apoptosis rate of lung tissue was detected by flow cytometry. The expression of Bcl-2 / Bax and Fas / FasL in lung tissue was detected by in situ hybridization. Results: Compared with Sh group, the apoptosis rate of lung cells and the expression of Bcl-2 / Bax and Fas / FasL genes in IR group and SF group were significantly increased (P <0.01). Compared with IR group, the apoptosis of lung tissue in SF group The rates of Bcl-2 / Bax and Fas / FasL gene expression were significantly decreased (P <0.05). Conclusion: Shenfu injection has a protective effect on lung ischemia-reperfusion injury, which is related to its inhibition on the expression of Bcl-2 / Bax and Fas / FasL in lung tissue and the decrease of apoptosis.