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以温度敏感型甘蓝型油菜雄性不育系160S为试验材料,并以可育油菜31C为对照,对160S育性转换过程中叶片和花药保护性酶的活性等进行了研究,研究中测定了160S在育性转换温度敏感时期超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)等抗氧化酶活性以及丙二醛(MDA)含量。结果显示,160S在育性转换期保护性酶活性降低,MDA过量积累;而作为对照的可育油菜31C保护性酶活性较高,MDA含量相对较低;以上差异和变化主要出现在花药中,表明不育系160S发生育性转换的一个重要因素是其花药中抗氧化酶活性系统产生了变化,使得活性氧自由基清除能力严重下降,从而进入活性氧自由基和MDA相互促进过量积累的不良循环。研究提示过量积累的活性氧自由基和MDA可能破坏了花药中细胞的膜结构,导致花药中花粉正常发育过程受阻而不能形成有活力的花粉母细胞,最终导致不育现象的发生。
Temperature-sensitive male sterile line Brassica napus L. 160S was used as experimental material, and fertile rapeseed 31C was used as a control to study the activity of protective enzymes of anther and anther during 160S fertility transformation. In this study, 160S During the temperature-sensitive period of fertility transformation, the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) MDA) content. The results showed that there was a decrease in protective enzyme activity and excessive accumulation of MDA in 160S during fertility transversion, while 31C protective enzyme activity and relatively low MDA content in fertile rapeseed as control were mainly found in anthers, The results showed that one of the important factors of fertility transformation in CMS line 160S was the change of its antioxidant enzyme system in anthers, leading to a serious decrease of reactive oxygen species scavenging ability, thus leading to the poor accumulation of reactive oxygen species and MDA cycle. The results suggest that excessive accumulation of reactive oxygen species (ROS) and malondialdehyde (MDA) may destroy the membrane structure of anther cells, resulting in the obstruction of normal pollen development in anthers and the inability to form viable pollen mother cells, leading to infertility.