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该研究以16份甘蔗骨干亲本为参照,对29份云南甘蔗创新种质进行SSR指纹图谱构建和遗传多样性分析,以明确创新种质与16份亲本间的遗传基础和多样性水平。结果表明:6对引物共扩增出104条带,其中101条为多态性条带,多态性条带比例为97.25%;45份材料的遗传相似性系数为0.235 3~0.891 3,平均值为0.563 3;其中16份甘蔗骨干亲本的遗传相似性系数为0.301 6~0.755 6,甘蔗创新种质与甘蔗骨干亲本的特异条带比例为14∶1,涵盖了割手密、大茎野生种、斑茅和滇蔗茅等基因源。根据骨干亲本间的相似性系数范围,在相似性系数为0.43处,可将种质分为6大类群,亲缘关系相对较远,适宜作为种质间的杂交利用。通过引物区分效率分析,6对引物扩增的多态信息量为0.967 9~0.975 8,其中MSSCIR21引物区分效率最高,利用MSSCIR21和SMC1047HA引物组合构建了云南甘蔗创新种质标准指纹图谱,在相似性系数为0.85处即可区分所有种质,图谱的鉴别准确率为100%,每份资源都有唯一的指纹图谱,可将29份创新种质和16份骨干亲本区分鉴别出来。该研究能够为后续杂交利用、种质鉴定和知识产权保护提供依据。
In this study, 16 sugarcane core parents were used as reference to construct 29 SSR fingerprinting and genetic diversity analysis of innovative sugarcane in Yunnan to identify the genetic basis and diversity of 16 germplasm and 16 parents. The results showed that a total of 104 bands were amplified by 6 pairs of primers, of which 101 were polymorphic bands and the percentage of polymorphic bands was 97.25%. The genetic similarity coefficient of 45 materials was 0.235 3 ~ 0.891 3, the average The value of which was 0.563 3. The genetic similarity coefficient of 16 main sugar cane backbone parents was 0.301 6 ~ 0.755 6. The ratio of specific bands of innovative sugarcane and sugarcane backbone parents was 14:1, Species, spot Mao and Yunnan Cimicifuga and other genetic sources. According to the range of similarity coefficient among key parents, the germplasm can be divided into 6 groups at the similarity coefficient of 0.43, which is relatively distant and suitable for the cross utilization between germplasms. According to the primer-specific efficiency analysis, the polymorphic information of six pairs of primers amplified from 0.967 9 to 0.975 8, MSSCIR21 primers were the most efficient. The standard fingerprinting of Yunnan sugarcane germplasm was constructed by using MSSCIR21 and SMC1047HA primer combinations, With a coefficient of 0.85, all germplasm can be distinguished, and the identification accuracy of the map is 100%. Each resource has a unique fingerprinting, and 29 innovative germplasm and 16 backbone parents can be distinguished. This study can provide the basis for subsequent hybridization, germplasm identification and intellectual property protection.