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目的:建立耐三苯氧胺(TAM)人乳腺癌的裸鼠移植瘤模型,为研究和治疗乳腺癌对TAM耐药提供研究工具。方法:采用雌激素受体阳性,对TAM耐药的人乳腺癌细胞系LCC2,接种于BALB/c裸鼠皮下,观察肿瘤生长趋势,用免疫组化方法进行鉴定。结果:在接种细胞数大于5×106/只时,Matrigel能够显著促进移植瘤的生长。肿瘤组织病理学检测证实为浸润性导管癌,且Pgp和Her-2为阳性表达。结论:该方法建立的耐TAM人乳腺癌移植瘤模型,周期短,成瘤率高,保留了与细胞系相同的肿瘤生物学特征。
OBJECTIVE: To establish a nude mouse xenograft model resistant to tamoxifen (TAM) human breast cancer and to provide a research tool for the study and treatment of breast cancer. Methods: Human esophageal cancer cell line LCC2 positive to estrogen receptor was inoculated into BALB / c nude mice subcutaneously to observe the tumor growth trend and identified by immunohistochemistry. Results: Matrigel could significantly promote the growth of xenografts when the number of inoculated cells was more than 5 × 106 / mouse. Tumor histopathology was confirmed as invasive ductal carcinoma with positive expression of Pgp and Her-2. CONCLUSION: The TAM-resistant human breast cancer xenograft model established by this method has a short cycle and a high rate of tumorigenesis and retains the same tumor biological characteristics as the cell line.