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目的食管癌的发生发展是多步骤、多基因变化的演化过程,本研究利用高通量的组织芯片技术,对食管癌组织及癌旁组织的p53、p16和环氧合酶(COX)-2蛋白异常表达进行分析,探讨其相关性及临床意义。方法利用组织芯片技术结合免疫组化法检测86例食管癌组织、40例癌旁组织中 p53、p16、COX-2蛋白的表达。结果食管癌组织中p53、COX-2的阳性表达率均显著高于癌旁组织(P< 0．05)。食管癌组织中p16阳性表达率为5．81％,癌旁组织中没有发现p16蛋白表达,差异无统计学意义。p53与p16、p53与COX-2、p16与COX-2蛋白表达均存在差异(P<0．05)。p53或COX-2表达阳性时组织芯片病理类型为癌性的概率增加,但p16、p53和COX-2三者不存在交互作用。结论 p53、 COX-2对预测和早期诊断食管癌具有重要意义。
Objective To investigate the occurrence and development of esophageal cancer, which is a multi-step and multi-gene evolution process. In this study, high-throughput tissue microarray was used to detect the expression of p53, p16 and cyclooxygenase-2 Abnormal expression of protein analysis to explore its relevance and clinical significance. Methods The expression of p53, p16 and COX-2 protein in 86 cases of esophageal cancer and 40 cases of paracancerous tissues were detected by tissue microarray and immunohistochemistry. Results The positive rates of p53 and COX-2 in esophageal cancer tissues were significantly higher than those in adjacent tissues (P <0.05). The positive expression rate of p16 in esophageal cancer tissues was 5.81%, but there was no p16 protein expression in adjacent tissues, the difference was not statistically significant. The expressions of p53, p16, p53 and COX-2, p16 and COX-2 were all significantly different (P <0.05). The positive rate of p53 or COX-2 expression was increased with the pathological type of cancer, but there was no interaction between p16, p53 and COX-2. Conclusions p53 and COX-2 are of great importance in the prediction and early diagnosis of esophageal cancer.