论文部分内容阅读
青果（ＣａｎａｒｉｕｍａｌｂｕｍＲａｅｕｓｃｈ）经去果核，脱脂，酸水解，水解液回收溶剂后碱萃取，萃取液酸化，再以溶剂提取，回收溶剂，经柱层析，洗脱，重结晶，得纯品，得率０．５％。经测试：ｍｐ＝２３９℃，Ｍ＝１７０，经１ＨＮＭＲ、１３ＣＮＭＲ、ＩＲ、ＭＳ检测，鉴定为没食子酸，理化性质与标准品或图谱基本一致。用ＥＬＩＳＡ法检测，抗ＨＢｓＡｇ／ＨＢｅＡｇ均有效，其效果与没食子酸对照品基本相同，认为没食子酸是青果中抗乙肝病毒的有效成分之一。
Canarium album Raeusch is denucleated, defatted, acid-hydrolyzed, the solvent is recovered in the hydrolyzate, the alkali is extracted, the extract is acidified, the solvent is then extracted, the solvent is recovered, column chromatography, elution, recrystallization, and pure product are obtained. The rate is 0.5%. Tested: mp=239°C, M=170, identified by 1H NMR, 13C NMR, IR and MS, identified as gallic acid, and the physicochemical properties are basically the same as those of the standard or the spectrum. The results of ELISA showed that the anti-HBsAg/HBeAg was effective and the effect was basically the same as that of the gallic acid control. Gallic acid was considered to be one of the active ingredients of anti-hepatitis B virus in the fruit.