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目的 观察bcl 2核酶对人肝癌细胞株SMMC 772 1细胞的作用 ,并检测 p16和 p2 1的表达情况。探讨bcl 2核酶在肝癌治疗中的意义。方法 经脂质体介导的方法 ,将PMTr neo (正向bcl 2核酶真核表达载体 )导入SMMC772 1细胞中。细胞克隆转移扩大培养后 ,采用TUNEL法、免疫组化技术结合图像分析 ,在检测SMMC772 1/PMTr neo细胞凋亡的同时 ,检测 p16 ,p2 1的表达。结果 与对照组相比较 ,在bcl 2核酶诱发SMMC772 1细胞发生凋亡的同时 ,p16和 p2 1基因的表达水平显著增高。结论 bcl 2核酶通过封闭bcl 2的表达可促进SMMC772 1细胞凋亡 ,同时伴发 p2 1和 p16表达水平的增高
Objective To observe the effect of bcl 2 ribozyme on human hepatocellular carcinoma cell line SMMC 772 1 and to detect the expression of p16 and p21. To explore the significance of bcl 2 ribozyme in the treatment of liver cancer. Methods PMTr neo (positive bcl-2 ribozyme eukaryotic expression vector) was transfected into SMMC772 1 cells by liposome-mediated method. After cell cloning, metastasis and expansion, the expression of p16 and p21 was detected by TUNEL method and immunohistochemistry combined with image analysis, while detecting the apoptosis of SMMC772 1 / PMTr neo cells. Results Compared with the control group, the expression of p16 and p21 genes was significantly increased in the apoptosis induced by bcl 2 ribozyme in SMMC772 1 cells. Conclusion bcl 2 ribozyme can promote the apoptosis of SMMC772 1 cells by blocking the expression of bcl 2, accompanied by an increase in the expressions of p21 and p16