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研究了 0～ 30 0mmol/LNaCl对大麦 (HordeumvulgareL .)幼苗生长速率、根系游离和结合态多胺含量以及多胺生物合成关键酶活性的影响。结果表明 ,在 0～ 2 0 0mmol/LNaCl处理下精氨酸脱羧酶 (ADC)、多胺氧化酶 (PAO)以及转谷酰胺酶 (TGase)活性明显提高 ,而在 30 0mmol/LNaCl处理下活性下降。与之对应 ,游离腐胺 (Put)含量随处理盐浓度的提高一直呈上升趋势 ,亚精胺 (Spd)和在根系内检测到的未知多胺 (PAx)在低浓度盐处理时含量上升 ,随盐浓度的提高含量下降。盐处理前后精胺 (Spm)含量变化不明显。低浓度盐处理时游离态 (Spd +PAx) /Put上升 ,随盐浓度的提高比值明显下降。结合态Put、Spd和PAx含量以及结合态多胺总量均在低浓度盐处理时上升 ,随盐浓度的提高含量明显下降。统计分析显示 ,大麦相对生长速率与游离态 (Spd +PAx) /Put和结合态多胺含量间均呈极显著正相关关系 ,与游离态多胺和结合态多胺的比值间均呈显著负相关关系 ,上述结果说明盐胁迫下大麦体内游离态Spd、PAx与Put以及结合态形式之间的平衡与大麦耐盐性关系密切 ,游离态Put向Spd、PAx以及结合态形式转化均有利于大麦耐盐性的提高。
The effects of 0 ~ 300 mmol / L NaCl on the growth of barley (Hordeum vulgare L.) seedlings, the contents of free radical and bound polyamines in roots and the activities of key enzymes involved in the biosynthesis of polyamines were studied. The results showed that the activity of arginine decarboxylase (ADC), polyamine oxidase (PAO) and transglutaminase (TGase) were significantly increased at 0 ~ 200 mmol / L NaCl treatment, while the activity of decline. Correspondingly, the content of free putrescine increased with the increasing of salt concentration. The contents of spermidine (Spd) and unknown polyamines (PAx) detected in roots increased at low salt concentration, With the increase of salt concentration, the content decreased. The content of spermine (Spm) did not change significantly before and after salt treatment. The concentration of free (Spd + PAx) / Put increased with low salt concentration, and decreased with salt concentration. The contents of bound Put, Spd and PAx, as well as the total amount of bound polyamines increased at low concentration of salt, and decreased with the increase of salt concentration. Statistical analysis showed that there was a significant positive correlation between the relative growth rate of barley and the content of free (Spd + PAx) / Put and bound polyamine, and negatively correlated with the ratios of free polyamine and bound polyamine , The above results showed that the balance between the free state Spd, PAx and Put and the bound form of barley was closely related to the salt tolerance of barley under salt stress. The transformation of free Put to Spd, PAx and bound forms was beneficial to the salt tolerance of barley improve.