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目的 了解抗病毒Ⅰ号中药有效部位 (AD1)对呼吸道合胞病毒 (RSV)的抑制作用。方法 采用细胞培养技术 ,以病毒唑为阳性对照药 ,比较观察AD1对RSV的抑制作用。结果 AD1的半数中毒浓度 (TC50 )为 2 5 .98mg/ml,抗RSV的半数有效浓度 (EC50 )为 0 .75 8mg/ml,治疗指数 (TI)为 34.2 9,且对RSV抑制作用存在明显的量效反应关系 (P <0 0 1)。感染RSV 2h、6h、12h后加药均可抑制RSV活性 (P <0 .0 1)。结论 AD1在Hela细胞中对RSV有明显抑制作用
Objective To understand the inhibitory effect of anti-virus I active site (AD1) on respiratory syncytial virus (RSV). Methods Cell culture techniques were used to compare the inhibitory effect of AD1 on RSV with ribavirin as a positive control drug. Results The half poison concentration (TC50) of AD1 was 25.98 mg/ml, the half effective concentration (EC50) of anti-RSV was 0.775 mg/ml, the therapeutic index (TI) was 34.2 9, and the inhibitory effect on RSV was obvious. The dose-response relationship (P < 0 01). RSV activity was inhibited after RSV 2 h, 6 h, and 12 h (P < 0.01). Conclusion AD1 has obvious inhibitory effect on RSV in Hela cells