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利用太谷核不育小麦和4D-4E 二体代换系杂交,通过中国春 phlb 突变体诱导部分同源配对,试图给太谷核不育小麦籽粒作出蓝色标记。观察到在 phlb 基因作用下,多价体频率加大,有可能使部分同源染色体4D 和4E 配对、交换,使太谷核不育基因(Tal)和4E 染色体上的蓝色胚乳基因连锁。从中得到少量中蓝色的全不育株行,其中 Tal 基因和蓝色胚乳基因的连锁,正在进行鉴定。
In this study, Taigu nuclear sterile wheat and 4D-4E substitution lines were crossed to induce the homologous pairing of Chinese phlb mutants. It was observed that under the action of phlb gene, the frequency of multivalent body increased, it is possible to pair and exchange partial homologous chromosomes 4D and 4E to link the Taigu nuclear sterile gene (Tal) with the blue endosperm gene on chromosome 4E. A small amount of medium-blue all-sterile plants were obtained, of which the linkage between the Tal gene and the blue endosperm gene was undergoing identification.