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The Daning River is a very important tributary in the Three Gorges Reservoir area. It is also a famous scenic spot. Anomalies appeared after water storage of the reservoir in June, 2003. In September, 2003, eutrophication monitoring in the 135-m backwater reach of the Daning River was conducted and the data were simply analyzed. The assessment result is that water body in the segment from Shuanglong to Longmen was mesotrophic or eutrophic. At the same time, the causes of its nutritional change were discussed, and counter-measures have been proposed.