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目的探讨外周血CD4+CD2+5CD1lo2w7调节性T细胞(Treg)在慢性心力衰竭(CHF)患者中的变化及其意义。方法采用流式细胞仪检测53例CHF患者和17例健康者(对照组)外周血Treg细胞占CD4+细胞的百分比(CD4+CD2+5CD1lo2w7细胞/CD4+T细胞),比较CHF不同心功能分级患者及对照组Treg细胞占CD4+细胞的百分比的变化。结果心功能NYHA分级Ⅲ～Ⅳ级患者Treg细胞占CD4+细胞的百分比[(2.47±0.36)%]低于心功能NYHA分级Ⅰ～Ⅱ级患者[(3.24±0.61)%],且两者与对照组[(5.23±0.94)%]相比,Treg细胞占CD4+细胞的百分比均降低,差异有统计学意义(P均<0.05)。相关性分析显示Treg细胞占CD4+细胞的百分比与脑钠肽呈负相关(r=-0.407,P<0.05)。结论慢性心力衰竭患者外周血Treg细胞比例降低;与慢性心力衰竭的严重程度有关;心力衰竭程度越重,Treg细胞比例越低;免疫激活以及炎症可能参与了其发生发展。
Objective To investigate the significance and significance of CD4 + CD2 + 5CD1lo2w7 regulatory T cells (Treg) in patients with chronic heart failure (CHF). Methods Flow cytometry was used to detect the percentage of Treg cells in CD4 (+ CD2 + 5CD1lo2w7 / CD4 + T cells) in 53 CHF patients and 17 healthy controls (control group) And control group Treg cells accounted for the percentage of CD4 + cells. Results The percentages of Treg cells in CD4 ~ + cells of NYHA class Ⅲ ~ Ⅳ patients [(2.47 ± 0.36)%] were lower than those of NYHA class Ⅰ ~ Ⅱ patients in cardiac function NYHA class Ⅲ ~ Ⅳ [(3.24 ± 0.61)%] Compared with the control group [(5.23 ± 0.94)%], the percentage of Treg cells in CD4 + cells decreased significantly (all P <0.05). Correlation analysis showed that the percentage of Treg cells in CD4 + cells was negatively correlated with brain natriuretic peptide (r = -0.407, P <0.05). Conclusion The proportion of Treg cells in peripheral blood of patients with chronic heart failure is lower than that of patients with chronic heart failure. It is related to the severity of chronic heart failure. The more severe heart failure, the lower the proportion of Treg cells. The activation of immune system and inflammation may be involved in the occurrence and development of Treg cells.