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应用免疫组织化学方法检测２２例肺鳞癌组织标本的ｐ５３及ｒａｓｐ２１表达，用显微图像分析仪测定阳性细胞百分率Ｐ／Ａ（％）和平均光密度（ＡＯＤ），并采用阳性水平指数（ｐｏｓｉｔｉｖｅｌｅｖｅｌｉｎｄｅｘ，ＰＬＩ）作为免疫组化反应水平指标，对癌基因蛋白进行比较研究。结果显示：ｐ５３表达阳性率为５０％，ｒａｓｐ２１表达阳性率为６３６％。ｐ５３蛋白表达在非角化型鳞癌组高于角化型鳞癌组；ｒａｓｐ２１的表达随组织分化程度的增高而增高，ｒａｓｐ２１与ｐ５３的表达水平无直线相关关系。
Immunohistochemistry was used to detect the expression of p53 and rasp21 in 22 specimens of squamous cell carcinoma of the lung. The percentage of positive cells P/A (%) and the average optical density (AOD) were measured with a microscopic image analyzer. The positive level index was used. , PLI) as an index of immunohistochemical reaction, a comparative study of oncogene proteins. The results showed that the positive rate of p53 expression was 50%, and the positive rate of rasp21 expression was 63.6%. The expression of p53 protein in non-keratinizing squamous cell carcinoma group was higher than that in keratinized squamous cell carcinoma group. The expression of rasp21 increased with the increase of tissue differentiation, and there was no linear correlation between the expression of rasp21 and p53.