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目的 研究 p16蛋白在胃癌和癌前病变 (不典型增生、慢性萎缩性胃炎及慢性消化性胃溃疡 )中的表达。方法 采用链霉菌抗生物素蛋白—过氧化物酶连接法 (S -P)。结果 p16蛋白阳性表达 :胃癌 47.5 4% (5 8/12 2 ) ,癌前病变 90 .0 0 % (72 /80 ) ,正常胃粘膜 96 .2 5 % (77/80 ) ,高分化腺癌 37.93 % (11/2 9) ,低分化腺癌 5 1.2 2 % (2 1/41) ,未分化癌 5 7.6 9% (15 /2 6 ) ,印戒细胞癌 6 2 .5 0 % (10 /16 ) ,粘液腺癌 10 .0 0 % (1/10 )。结论 p16蛋白表达 :胃癌组与正常对照组及癌前病变组间的差异均有显著性意义 (P <0 .0 5 ) ;粘液腺癌与低分化腺癌、未分化腺癌及印戒细胞癌之间的差异也均有显著性意义 (P <0 .0 5 )。
Objective To study the expression of p16 protein in gastric cancer and precancerous lesions (dysplasia, chronic atrophic gastritis and chronic peptic ulcer). Methods Streptomyces avidin-peroxidase ligation (S-P) was used. Results The positive expression of p16 protein in gastric cancer was 47.5% (58/122), 90.0% (72/80) in precancerous lesions, 96.25% (77/80) in normal gastric mucosa, well-differentiated adenocarcinoma 37.93% (11/2 9), poorly differentiated adenocarcinoma 5 1.2 2% (2 1/41), undifferentiated carcinoma 5 7.69% (15/2 6), signet ring cell carcinoma 62.5% / 16), mucinous adenocarcinoma 10.0% (1/10). Conclusion The expression of p16 protein is significantly different between gastric cancer group and normal control group and precancerous lesions group (P <0.05); mucinous adenocarcinoma and poorly differentiated adenocarcinoma, undifferentiated adenocarcinoma and signet ring cell The differences between the cancers were also significant (P <0.05).