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目的为了解人芽囊原虫对外界条件的适应性,观察培养条件改变对其形态和繁殖方式的影响. 方法从腹泻患者粪便中检查并分离培养获得人芽囊原虫,分别用locke氏-鸡蛋双相培养基(LES)和改良1640培养基(RPMI-1640)培养,改变培养条件,观察人芽囊原虫形态和繁殖方式. 结果在两种培养基中均可见到人芽囊原虫的四种主要形态:空泡型、颗粒型、阿米巴型和包囊型,其繁殖方式以二分裂为主,可见少量出芽生殖.当从LES培养基突然变为RPMI-1640培养基时,人芽囊原虫出现短时间(48～72h)内生长停滞,随后出现多种形式的繁殖方式,可见较多的二分裂、出芽生殖、三分裂、多分裂、裂体生殖,持续24h后恢复为二分裂为主. 结论培养条件改变能够引起人芽囊原虫繁殖方式的改变,出现短暂性多样化繁殖方式,人芽囊原虫对环境的适应能力很强.“,”Objective To understand the adaptability of Blastocystis hominis (B. hominis ) to environment, observe effect of culture condition change on reproductive mode of B. hominis. Methods B. hominis was isolated from patient with diarrhea and cultured in different culture medium including Locke-egg-serum medium culture (LES) and modified RPMI-1640 culture medium (RPMI-1640). When culture condition was changed between LES and RPMI-1640, morphous and reproductive modes were observed. Results All of 4 form including vacuolar form, granular form, ameba form and cyst form were found in each culture medium, separately, and main reproductive mode is binary fission, a few of gemmation was exist. When culture condition was changed abruptly from LES medium to RPMI-1640 medium, different reproductive mode including binary fission, ternary fission,multiple fission and gemmation were found after growth retardation of 24 ～ 72 hours. This phenomenon continued less than 24 hours and binary fission remained reproductive mode then. Conclusion Reproductive mode of B. hominis can be effected by culture condition change, temporary diversified reproductive mode appear. B. hominis has very strange adaptability to environment.