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在调种引种过程中,有时由于任务重,时间紧迫,或在不具备发芽试验条件下,短期内又急需了解种子发芽率,可用生物化学速测法测定种子的生活力(种子发芽的潜在能力)来代替发芽试验,间接了解种子的发芽率。常用的方法有:一、四唑染色法[药剂及原理]四唑盐类中有多种试剂可用来测定种子生活力,最常用的是“2、3、5——氯化三苯基四氮唑”,为白色或淡黄色粉剂,有毒,对光敏感性强,宜装在综色瓶内放于暗处。
In the process of seed introduction and introduction, the seed germination rate may be urgently needed in a short period due to heavy workload, time constraints or without germination test, and the viability of the seed can be determined by the biochemical rapid test (the potential of seed germination ) Instead of germination test, an indirect understanding of the germination rate of the seed. Commonly used methods are: First, tetrazolium staining [Pharmacy and principles] There are many reagents tetrazolium salts can be used to determine the viability of the seed, the most commonly used is “2,3,5 - triphenyl chloride Azole ”, white or light yellow powder, toxic, sensitive to light, should be loaded in the bottle of synthetic put in the dark.