长期继代培养的愈伤组织,不仅是建立细胞无性繁殖系和作为种质资源保存的一种材料,也是细胞突变育种的理想试材。这主要是材料一致,容易处理,不受季节和植物发育阶段的限制,任何时候都可以进行试验,给研究工作提供了很多方便,避免了对种子和接穗作辐射诱变和化学诱变时容易形成的大量嵌合体,提高了诱变频率。 但是,除必须提高长期继代培养愈伤组织的增殖能力外,还要解决愈伤组织再生植株的分化率,以及植株的染色体倍性和其它遗传性状是否发生变化的问题。本试验试图通过对长期继代培养的柳橙(Citrus sinensis Osbeck)胚的愈伤组织进行研究,以对上述问题进行探讨。 Long-term subculture of callus, not only to establish cell clones and as a germplasm preservation of a material, but also cell mutation breeding ideal material. This is primarily material-consistent, easy to handle, and not susceptible to seasonal and vegetative developmental stages and can be tested at any time, providing research convenience with ease and avoiding the need for radiation and chemical mutagenesis of seeds and scions The formation of a large number of chimerism, increased mutagenic frequency. However, in addition to the need to improve long-term subculture of callus proliferation, but also to resolve the differentiation rate of callus regeneration plants, and plant chromosome ploidy and other genetic changes whether the problem. This study attempts to investigate the above problems by studying the callus of long-term subcultured Citrus sinensis Osbeck embryos.